Fig. 1: Epithelium and bone marrow minimally contribute to pancreatic cancer associated fibroblasts. | Nature Communications

Fig. 1: Epithelium and bone marrow minimally contribute to pancreatic cancer associated fibroblasts.

From: The splanchnic mesenchyme is the tissue of origin for pancreatic fibroblasts during homeostasis and tumorigenesis

Fig. 1

a Schematic of the KPFG mouse model. b–d Co-immunostaining in the KPFG pancreas and quantification of cells that are either single positive or double positive for defined markers. n = 7 mice for PDGFRβ analysis; n = 8 mice for αSMA analysis. e–h Flow cytometry analysis of dissociated KPFG pancreas and quantification of GFP + cells within populations defined by markers. n = 4 mice for GFP-; n = 3 mice for GFP + (normal); n = 9 mice for GFP + (KPFG). ****p < 0.0001; ns for PDPN: p = 0.996; ns for PDGFRα: p = 0.923. i Schematic of the bone marrow transplant (BMT) experiment. j–l Co-immunostaining in KPF pancreas transplanted with GFP + bone marrow and quantification of cells that are either single positive or double positive for defined markers. n = 5 mice for PDGFRβ analysis; n = 3 mice for αSMA analysis. m–p Flow cytometry analysis of pancreas from KPF mice transplanted with GFP + bone marrow and quantification of GFP + cells within populations defined by markers. n = 4 non-transplanted mice; n = 5 transplanted normal mice; n = 6 transplanted KPF mice. Data are mean ± SD. ****p < 0.0001; ns for PDPN: p = 0.744; ns for PDGFRα: p = 0.340. h, p Two-way ANOVA test with Tukey’s multiple comparisons was performed. ns, not significant. Scale bars: 30 μm. Source data are provided as a Source data file.

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