Fig. 2: Adipose-specific deletion of Parkin ameliorates high-fat diet and aging-induced obesity and glucose tolerance.

a Body weight of HFD-fed Control f/f and Parkin^Adi mice (n = 8 mice per genotype) [Two-way ANOVA F(1,14) = 29.51, p < 0.0001, 95%CI = 3.887–6.236]. b Body consumption of Control f/f and Parkin^Adi mice following a 9-week HFD (n = 6 mice for Control f/f group, n = 8 mice for Parkin^Adi group) [Unpaired Student’s t test two-tailed. For fat mass Control f/f-HFD vs. Parkin^Adi-HFD, p = 0.0246, 95%CI = −10.68 to −0.8768, R squared = 0.3547]. c Glucose tolerance test on 6 weeks of HFD-fed Control f/f and Parkin^Adi mice (n = 7 mice per genotype) [Two-way ANOVA F(1,12) = 6.477, p = 0.0257, 95%CI = 5.134–66.21]. d Insulin tolerance test on 7 weeks of HFD-fed Control f/f and Parkin^Adi mice (n = 5 mice per genotype) [Two-way ANOVA F(1,8) = 7.594, p = 0.0248, 95%CI = 5.337–60.06]. e H&E-stained sections of eWAT, iWAT, and BAT from 9 weeks HFD-fed mice; scale bars = 50 μm (n = 5 mice per genotype). Adipocyte area in eWAT was shown in box and whiskers (Control f/f: minimum value 0.0025, average value 90.50, maximum value 488.2454; Parkin^Adi: minimum value 0.0025, average value 62.48, maximum value 267.0925) [Unpaired Student’s t test two-tailed. p < 0.0001, 95%CI = −35.78 to −20.24, R squared = 0.0544]. f Volcano plots and enrichment analysis of RNA sequencing results from eWAT of HFD-fed Parkin^Adi vs. Control f/f mice (n = 4 mice per genotype). g Immunofluorescent staining of F4/80, Perilipin 1, and DAPI in the eWAT of HFD-fed Control f/f and Parkin^Adi mice (n = 3 mice per genotype) [Unpaired Student’s t test two-tailed. p = 0.0020, 95%CI = −3.519 to −1.558, R squared = 0.9281]. Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, **** p < 0.0001. Source data are provided as a Source Data file.