Fig. 3: In vivo 3D calcium imaging of neuronal structures.
From: Optical gearbox enabled versatile multiscale high-throughput multiphoton functional imaging
a Experimental configuration of the 3D calcium recording. NA numerical aperture, ROI region of interest. b Maximum intensity projection of the imaging volume along the axial direction. PB primary branch, SB secondary branch, MIP maximum intensity projection. The experiment was repeated independently in 4 mice with similar results. c 2D images at various depths. d Calcium transients from the various regions of the neuronal structures.
