Fig. 4: High-speed large-scale functional blood flow imaging.
From: Optical gearbox enabled versatile multiscale high-throughput multiphoton functional imaging
a Experimental configuration with the whisker stimulation and fluorophore injection. FITC fluorescein isothiocyanate. b Large field imaging of the blood vessel network. c Imaging fluorescence beads in fast blood flow with a 200 Hz frame rate. d Imaging fluorescence beads in slow blood flow with a 200 Hz frame rate. e Top view of the large area cranial window and the regions of interest. f Blood flow speed before and during the whisker stimulation across the five regions of interest. Data are represented as mean ± standard error of the mean. ***P = 0.0007. Stim stimulation, ROI region of interest. g Statistical analysis of the blood flow speed variation. For boxplots, minima and maxima are shown as the bounds of whiskers, and the centile, upper and lower quartiles are shown as the middle, top, and bottom lines of the box. **P (ROI 1 vs 3) = 0.0011, *P (ROI 2 vs 3) = 0.0135, *P (ROI 3 vs 5) = 0.04. One-way ANOVA Tukey’s multiple comparisons test in (f and g). n = 108/23, 73/18, 75/11, 61/19, 138/29 (rest/stimulation) speed measurements in ROI 1-5 from one animal, respectively. The experiment was repeated independently in 8 mice with similar results.
