Fig. 1: Monocytosis and enhanced mTORC1 signaling in IL-1-mediated inflammation.
From: mTORC1 links pathology in experimental models of Still’s disease and macrophage activation syndrome
a Complete blood count in BALBc (n = 5) and IL1rn−/− mice (n = 6 per group), and spleen size in BALBc (n = 6) and IL1rn−/− mice (n = 8). b Flow cytometry quantification of circulating Ly6Chi monocytes in BALBc and IL1rn−/− mice at baseline (n = 10 per group) and (c) after anakinra treatment for 2 weeks (WT, n = 3; IL1rn−/− PBS, n = 8; IL1rn−/− + anakinra, n = 6). d Peripheral blood monocyte and lymphocyte count in sJIA patients pre-treatment and 2 weeks after initiation of anakinra (n = 8). e t-SNE display of leukocyte clustering derived from single-cell RNA-seq of peripheral blood cells from BALBc and IL1rn−/− mice (4 mice pooled per group). f Volcano plot of differentially expressed genes in Ly6Chi monocytes of WT and IL1rn−/− mice. g Density plot of Hallmark mTORC1 gene set expression using AddmoduleScore. h Cluster plot of gene set enrichment in peripheral blood leukocyte subsets of BALBc and IL1rn−/− mice based on single-cell RNA-seq (4 mice pooled per group). i Phospho-flow analysis of mTOR substrates in bone marrow Ly6Chi monocytes from WT and IL1rn−/− mice at baseline (n = 6 per group) and (j) after daily anakinra treatment for 2 weeks (n = 6 per group for phospho-S6 and phospho-4EBP1; n = 3 for phosphor-Akt). Data in (a, b, c, i, and j) were pooled from 2 to 3 independent experiments. Mice were 8–9 weeks old for all experiments. Statistical analyses (all two-sided): Mann–Whitney U test (a, b, c, d, i, j); Wilcoxon signed-rank test with Bonferroni correction (f). Median and error bars representing interquartile range are displayed in (a, b, c, i, j). Source data are provided as a Source Data file.
