Fig. 6: The effects of Batf3−/− cDC1-deficient on tumor microenvironment and CD39i efficacy.
From: Single cell sequencing reveals that CD39 inhibition mediates changes to the tumor microenvironment
A Single cells from WT and Batf3−/− cDC1-deficient mice treated with or without CD39i were clustered into 10 major clusters, 3 subcutaneous tumors from 3 mice in each group mixed 1:1:1. B The proportions of 8 major clusters without stromal cells and epithelial cells. C The DCs were clustered into 5 clusters based on classical markers. D Ratios of observed to expected cell numbers (Ro/e) for each DC subcluster in different groups, Ro/e > 1.1 was considered as enriched in the group. E Compared to WT mice, mDC from Batf3−/− mice expressed more Ccl17 and Ccl22 and less Il12b. F Lymphocytes were clustered into 15 clusters. G CD39i treatment significantly increased the proportions of proliferating CD8 + T cells and NK cells, but decreased the proportion of Treg and Th cells in Batf3+/+ mice. In Batf3−/− mice, only a slight increase in NK cells was observed. H, I CD39i was not responsive to mononuclear macrophages in both Batf3+/+ mice and Batf3−/− mice.
