Fig. 4: On-target activities of SuperFi prime editor and nickase activities of D10A and H840A nickase variants in mammalian cells. | Nature Communications

Fig. 4: On-target activities of SuperFi prime editor and nickase activities of D10A and H840A nickase variants in mammalian cells.

From: SuperFi-Cas9 exhibits remarkable fidelity but severely reduced activity yet works effectively with ABE8e

Fig. 4

a Prime editing activity of SuperFi. Data are from Supplementary Fig. 10d–e. Results are presented on a scatter dot plot, the median and interquartile ranges are shown; data points are plotted as open circles representing the means of triplicates for n = 13 target sites. b (left) On-target disruption activities of nickase SpCas9 variants with paired sgRNAs. The heatmap shows the mean on-target modifications (indels) of three parallel transfections. (right) +/− indicate the activity of the nuclease variants with the corresponding sgRNAs. a, b Summary of target and primer sequences, EGFP disruption and NGS data are reported in Supplementary Data 1–3.

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