Fig. 1: A cell-intrinsic function for CD1d in the regulation of TLR responses.

a, b Peritoneal macrophages (pMacs) were obtained from WT and CD1d-KO mice. a Representative flow cytometry of WT (blue) and CD1d-KO (red) pMacs showing expression of the depicted markers in steady state. b Secretion of IFN-β (left, n = 2–3), normalised concentration of secreted cytokines (fold over WT, middle, n = 4–9) and gene expression (right, n = 4–5) in WT and CD1d-KO pMacs cultured with LPS for 6 h. *p < 0.05; **p < 0.01, 2-way ANOVA (left), one-sample (middle) or two-tailed paired t-test (right). n.s. = non-stimulated. c Mixed BM chimeras (WT:CD1d-KO; 50:50) were generated by co-transferring WT (CD45.1⁺) and CD1d-KO (CD45.2⁺) BM into irradiated recipients (CD45.1⁺CD45.2⁺). WT and KO pMacs were sort-purified from chimeric mice and stimulated with LPS. Normalised concentration of IL-6 (fold over WT, left, n = 4) and gene expression measured by qPCR (right, n = 4) are shown. *p < 0.05; ***p < 0.001, one-sample (left) or two-tailed paired t-test (right). n.s. = non stimulated. d GM-CSF cultured bone-marrow derived cells (BMDCs) were generated from WT and CD1d-KO mice. Representative flow cytometry of WT (blue) and CD1d-KO (red) BMDCs showing expression of CD1d in steady state. Secretion of IFN-β (left, n = 3), normalised concentration of IFN-β (fold over WT, middle, n = 4–11) and gene expression (right, n = 4–8) in WT and CD1d-KO BMDCs stimulated for 6 h as indicated (2 ng/ml LPS; 0.2 μM CpG; 5 μg/ml poly I:C). *p < 0.05; **p < 0.01; ***p < 0.001, 2-way ANOVA (left), one-sample (middle) or two-tailed paired t-test (right). n.s. = non stimulated. e WT mice were pre-treated with clodronate liposomes and reconstituted with WT or CD1d-KO BMDCs. Mice were injected with LPS and body temperature (left) or cytokine concentration in the blood (right) were measured (n = 5–7). *p < 0.05; **p < 0.01, two-tailed unpaired t-test. Bars in all graphs represent mean +/− SEM. Values for n represent biologically independent samples (as shown by the number of data points in each graph). n = cells isolated from individual mice (a–d) or mice per group (e). Source data are provided as a Source Data file.