Fig. 4: Metabolic rewiring in CD1d-KO cells.
From: CD1d-dependent rewiring of lipid metabolism in macrophages regulates innate immune responses
a Cholesterol and cholesteryl ester contents in pMacs detected using liquid chromatography-tandem mass spectrometry (LC/MS/MS) were normalized by cell numbers (n = 5). b Flow cytometry-based readout of cholesterol content by Filipin staining (n = 5). c pMacs were isolated from WT and KO mice and stained with cholera toxin B (CtB; green) and F4/80 (red). Bars represent mean +/− SEM for corrected total cell fluorescence (n = 161 WT; 261 CD1d-KO). Data pooled from 2 independent experiments. Scale bar is 20 μm. d Flow cytometry-based readout of Nile Red staining (n = 4). e Uptake of DiI-acLDL in pMacs isolated from WT or CD1d-KO mice (n = 7). *p < 0.05, unpaired two-tailed t-test. Bars in all graphs represent mean +/− SEM. Values for n represent biologically independent samples (as shown by the number of data points in each graph). n = cells isolated from individual mice (a, b, d, e) or cells (c). Source data are provided as a Source Data file.
