Fig. 6: CD1d regulates CD36 internalization and lipid uptake.

a qPCR expression data of Cd36 and Msr1 in WT and CD1d-KO cells (n = 4–6). b Western-blots and quantification for CD36 and β-actin in WT and CD1d-KO BMDCs (n = 3). c Flow cytometry images and quantification of Mean fluorescence intensity (MFI) for CD36 and MSR1 in WT and CD1d-KO cells (n = 3–13). *p < 0.05, ***p < 0.001, one-sample t-test. d Confocal microscopy images showing staining of CD1d (red), CD36 (green) and DAPI (blue) for WT (n = 123) and CD1d-KO BMDCs (n = 91). Graphs show fluorescence intensity for CD1d and CD36 (left) and Pearson correlation coefficient (right). Scale bar is 20 μm. ****p < 0.0001 two-tailed unpaired t-test. e PLA was performed in WT and CD1d-KO BMDCs for CD1d and CD36. Representative images of PLA (yellow) and DAPI (blue) and quantification of PLA (puncta per cell) are shown (n = 162–305 cells). Scale bar is 7 μm. ****p < 0.0001 two-tailed unpaired t-test. f Flow-cytometry analyses of surface CD36 in WT and CD1d-KO pMacs after incubation with oxLDL (left). MFI for CD36 (middle) or values relative to control (untreated) cells (right) are shown (n = 6). **p < 0.01; ***p < 0.001; ****p < 0.0001, 2-way ANOVA. g Flow-cytometry analyses of CD36 in WT pMacs pre-treated with αCD1d or isotype (iso) and incubated with oxLDL (n = 9). CD36 levels are shown relative to control (untreated cells + isotype). *p < 0.05; paired two-tailed t-test. h Uptake of DiI-acLDL in pMacs pre-treated with αCD1d or isotype (n = 4). **p < 0.01, unpaired two-tailed t-test. i BMDCs were cultured with αCD1d or isotype. qPCR data show relative expression of the depicted genes (n = 9). **p < 0.01 one-sample t-test. j BMDCs were cultured with αCD1d or isotype (iso) and stimulated with LPS (n = 5–9). Expression of the depicted genes was measured by qPCR. *p < 0.05, **p < 0.01 one-sample t-test. k Uptake of DiI-acLDL in HEK293T cells transfected with CD1d, mutant CD1d (CD1d-TD or CD1d-Y) and/or CD36 (n = 4). Some samples were incubated with αCD1d as indicated. *p < 0.05; **p < 0.01; ***p < 0.001, one-way ANOVA. Bars in all graphs represent mean +/− SEM. Values for n represent biologically independent samples (as shown by the number of data points in each graph). n = cells isolated from individual mice (a–c, f–j), cells (d, e) or transfections (k). Source data are provided as a Source Data file.