Fig. 4: TEAD–YAP/TAZ blockade promotes transcriptional activation of PIK3C2B and SOX4 mediated by VGLL3.

a Volcano plot of RNA-seq results of MDA-MB-231 cells treated with MGH-CP1. b Venn diagram of significantly upregulated genes treated with MGH-CP1 and siYAP/TAZ in various cancer cell lines. c Heatmap of 51 genes commonly upregulated with TEAD–YAP/TAZ blockade. Top 10 genes with the highest fold change were shown in the box. d Pearson correlation of PIK3C2B transcriptional level against YAP signature in tumor cells. Transcriptional level of YAP, TAZ, Cyr61, CTGF, ANKRD1 (e), PIK3C2B and SOX4 (f) were examined in DLD1, Huh7, HUTU80, H1299, H226 and MDA-MB-231 cells with siYAP/TAZ knockdown. All the gene expression levels of siYAP/TAZ treated samples were normalized to siControl (dashline). (n = 3 biological repeats). siYT, siYAP/TAZ. g PIK3C2B and SOX4 transcriptional levels in Huh7, MDA-MB-231, HCT116, DLD1 and HCT15 treated with MGH-CP1 treatment were assessed by qPCR (n = 3 biological repeats). h H226 cells were treated with MGH-CP1 and K-975, PIK3C2B and SOX4 expression levels were shown (n = 3 biological repeats). i HEK293A cells were overexpressed with YAP or TAZ, transcriptional levels of YAP, TAZ, Cyr61, CTGF, ANKRD1, PIK3C2B and SOX4 were assessed (n = 3 biological repeats). j PIK3C2B and SOX4 transcriptional levels in HEK293A cells with overexpression of human VGLL3 (n = 3 biological repeats). k HEK293A cells were treated with Control siRNA or VGLL3 siRNA in the presence of vehicle control of MGH-CP1, VGLL3, PIK3C2B and SOX4 expression levels were shown (n = 3 biological repeats). l VGLL3 was silenced with siRNA in MDA-MB-231, and VGLL3, PIK3C2B and SOX4 transcriptional levels were determined (n = 3 biological repeats). Data are represented as mean ± S.E.M. P values were determined using two-tailed t-tests. Source data are provided as a Source Data file.