Fig. 1: N2 fixation rates and community composition by station.

Map inset shows stations (S) sampled across the North Pacific Subtropical Gyre. Bar chart shows community N2 fixation rates averages under natural-light (yellow) and all-dark (dark grey) conditions derived from biological triplicates. Individual N2 fixation rates values are shown as black circles for each station and light treatment. Error bars are the standard deviations of the averages (n = 3). The limit of detection (LOD) for the N2 fixation rates are shown with an X. Stations where the X is above the average are below the LOD but above the minimum quantifiable rate (MQR), all-dark values at S17 and S20 were below LOD and MQR (red), no data were available for S23 all-dark incubation. The lower pie charts show the relative proportion of cyanobacterial (green) and NCD (light grey) nifH sequences at each station. Source data are provided as a Source Data file.