Fig. 2: The effect of ocean acidification on cellular metabolite, pigment, and gene transcription of P-limited T. erythraeum.
a PolyP (femto-equivalents of the standard per cell), NAD(H) and NADP(H) concentrations, and NAD(H):NADP(H) ratios of P-limited T. erythraeum under ambient and acidified conditions. b Percentage change (acidified normalized to ambient condition) of gene transcription of NAD kinase (cyan) and proteins involved in Chla synthesis (green), PSI (purple), and ATP synthesis (yellow). Solid bars denote transcriptomic analysis data, and open bars denote RT-qPCR analysis data (Supplementary Tables 4 and 5). c cellular Chla, ATP, and Glu concentrations of P-limited T. erythraeum under ambient and acidified conditions. In a, c, data are presented as mean values + SD (n = 3 biologically independent samples, except for Chla n = 14 and 15 under ambient and acidified conditions, respectively), and dots are corresponding data points of the replicates. Asterisks denote significant changes in polyP (p = 0.007), NAD(H):NADP(H) ratios (p = 0.003), Chla (p < 0.001), ATP (p = 0.036), and Glu (p = 0.031) under acidified conditions compared with ambient conditions (two-tailed paired Student’s t-test). In b, data are mean values of three biological replicates (n = 3 biologically independent samples), and asterisks denote significant changes (p < 0.05) in gene transcription in response to acidification (for the transcriptomic data analysis, differential expression was analyzed using the DESeq2 R package54 and the p-values were adjusted using the Benjamini and Hochberg’s approach; for RT-qPCR data analysis, two-tailed paired Student’s t-test). Source data are provided as a Source Data file.
