Fig. 5: Prophylactic blockade of PGE2 signaling through the EP2 receptor improves survival to IAV infection in aged mice. | Nature Communications

Fig. 5: Prophylactic blockade of PGE2 signaling through the EP2 receptor improves survival to IAV infection in aged mice.

From: Age-induced prostaglandin E2 impairs mitochondrial fitness and increases mortality to influenza infection

Fig. 5

a, b Aged (18–22 months) female C57BL/6 mice were given 7 daily i.p. injections of the EP2 antagonist starting 7 days before infection or 11 daily i.p. injections of the EP2 and EP4 antagonists starting 7 days before infection. The mice were then infected with 400 pfu of PR8 IAV intranasally as depicted in (a). Survival of mice (b) was tracked daily. n = 14 for Veh Control, n = 6 for EP2 Antg, and n = 9 for EP2 + EP4 Antg. c, d Aged mice were given clodronate-loaded liposomes followed by an adoptive transfer of WT or EP2 KO AMs. 4 weeks following the adoptive transfer, the mice were given EP2 antagonist or vehicle control for 7 days prior to infection with 400 pfu of PR8 IAV intranasally as depicted in (c). Survival of mice (d) was tracked daily. n = 8 for WT AMs+EP2 Antg. n = 9 for EP2 KO AMs+ Veh Control and WT AMs + Veh Control. n = 10 for EP2 KO AMs+ EP2 Antg. For panels b and d, survival differences were statistically determined by two-tailed Gehan–Breslow–Wilcoxon test. Schematics created in BioRender. Source data are provided as a Source Data file.

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