Fig. 1: Reconstitution of the human Rix1 complex.
From: Cryo-EM reveals the architecture of the PELP1-WDR18 molecular scaffold
a Schematic representation of human Rix1 complex members PELP1 (teal), WDR18 (orange), TEX10 (pink), and SENP3 (blue) with amino acid boundaries. b Western blot and total protein stain from SDS-PAGE of sequentially built reconstituted Rix1 complexes isolated by co-IP of N-terminal FLAG tagged PELP1 constructs. Lanes 1-5 are Rix1 complexes built over full-length PELP1 (1-1130aa) and lanes 6-10 are complexes built over PELP1 Rix1 domain (1-642aa). Teal triangle denotes band representing C-terminal truncated PELP1 originating from full-length. Colored circles denote bands representing like-colored Rix1 complex members in (a). Red asterisks denote 3X FLAG peptide band. c Western blot from SDS-PAGE of the human Rix1 complexes affinity isolated using PELP1 C-terminal truncations for mapping of TEX10 and SENP3 binding regions within the PELP1 C-terminus. d Cartoon representation of human Rix1 complex members interacting with proteins/regions determined in (b, c). co-IP and Western blot experiments in (b, c) were performed at least two times with reproducible results. Uncropped gel images available in Source Data file.
