Fig. 1: Overall structure of the EARS1–IARS1 complex.
From: Regulation of BRCA1 stability through the tandem UBX domains of isoleucyl-tRNA synthetase 1
a A list of MSC components identified by mass spectrometry. Total spectrum count of identified proteins interacting with IARS1ΔC, IARS1 UNE-I, or IARS1 WT is shown. b Affinity pull-down analysis of the interaction between EARS1 and IARS1. The input (I) and eluate (E) fractions were analyzed by SDS-PAGE and Coomassie Blue staining. Black and red arrowheads indicate eluted His-IARS1 and GST-EARS1, respectively. The data are representative of three independent experiments. Source data are provided as a Source Data file. c A schematic illustration of the architectures of EARS1 and IARS1. d Structure of the EARS1–IARS1 complex. The core regions of EARS1 (light blue) and IARS1 UNE-I (KH, green; UBX1, orange; and UBX2, pink) are shown at the top and bottom of the image, respectively. Close-up views of the boxed regions are shown in Fig. 2a–c. e–g Structures of UBX1 e, UBX2 f, and Ub g.
