Fig. 1: Infection dynamics in blood and gonadal adipose tissue.
From: Slow growing behavior in African trypanosomes during adipose tissue colonization
a C57BL/6J mice were infected with a pleomorphic T. brucei GFP::PAD1utr stumpy reporter cell-line. The number of parasites per milligram of tissue was quantified by qPCR (red and yellow lines and dots) and the percentage of stumpy forms was estimated by flow cytometry as the proportion of GFP-positive parasites (red and yellow bars). Parasite density: n = 3 independent experiments for all time points, except for day 21 in which n = 4 independent experiments. Percentage of stumpy forms: n = 3 independent experiments for all time points, except for day 6 in which n = 7 independent experiments and day 7 in which n = 5 independent experiments. The dashed lines correspond to noncontiguous time points and the full lines to contiguous time points. Error bars represent the standard error of the mean. Occasionally, the parasite density was too low which resulted in n = 2. b Percentage of stumpy forms in blood (red circles) and gonadal adipose tissue (yellow circles) for the 17 studied time points (n = 38 BSFs and n = 40 ATFs pooled from all independent experiments from all time points). Gray lines represent the average percentage of stumpy forms and error bars the standard deviation, two-sided Wilcoxon-signed rank test, p = 1.205e-8. c Percentage of stumpy forms in blood and gonadal adipose tissue of six mice sacrificed at day 6 of infection. Source Data are provided as a Source Data file.
