Fig. 3: Proteome analysis and protein synthesis of parasites isolated from blood and gonadal adipose tissue.
From: Slow growing behavior in African trypanosomes during adipose tissue colonization
a Representative Volcano plot of 1 out of the 1000 performed imputations with the 2693 protein groups detected by mass spectrometry in the sum of BSFs and ATFs 8 data sets. Red and yellow dots indicate protein groups significantly upregulated in BSFs and ATFs, respectively. Proteins were obtained from Lister 427 parasites isolated from a pool of 5–6 animals infected for 5 days (n = 4 independent experiments). b Heat map representing the z-score abundances of the differentially expressed protein groups. Each column corresponds to one independent pool of mice. c Enriched GO terms (Biological Process) of the differentially upregulated (yellow circles) and downregulated (red circles) proteins according to gene ratio, using a False Discovery Rate corrected p-value cut-off of 0.05 (two-sided Fisher’s exact test). Each dot corresponds to a given GO term: the size represents the number of differentially expressed protein groups and the color the respective p-value. d Fluxogram of the experimental procedure used to quantify protein synthesis in Lister 427 parasites isolated from blood and gonadal adipose tissue 5 days post-infection. e Percentage of HPG internalized by ATFs (yellow circles) normalized to the percentage of internalization of the BSFs (red circles) (n = 6 independent experiments). Gray lines represent the average percentage of HPG incorporation (normalized to BSFs) and the error bars represent the standard deviation. f Mean Fluorescence Intensity of the HPG incorporated for 30 min by the isolated BSFs and ATFs for experiments A, B, C, D, E, and F (n = 6 independent experiments). Two-sided Wilcoxon-signed rank test, p = 0.03125. Source Data are provided as a Source Data file.
