Fig. 6: Prior LPS exposure diminishes transcriptional initiation but has little effect on export rates.
From: Kinetics of mRNA nuclear export regulate innate immune response gene expression
A Comparison of observed basal expression (top) and max fold change (bottom) at the chromatin level. Tolerization increases basal expression and decreases induced expression for most genes. B Heatmap of fold change in the naive condition and tolerized condition. We observe in all fractions that the induction is dimmed in the tolerized condition. C Distribution of fit quality for tolerized and naive macrophages. The fit quality for the tolerized macrophage is not as good as for the naive (heavier right tail). Source data are provided as a Source Data file. D Comparison of post-transcriptional parameters in naive and tolerized cells. We observe that k1’ and k2 are not well-defined, as in naive macrophages. However, k2’ and kcyto_deg are well-defined and very similar between to those in naive condition. E Comparison of the composite parameters, effective transport rate (k1’k2’/k2), and export efficiency (k2’/k2), between naive and tolerized conditions. These parameters have similar values for most genes, even if less well-defined in the tolerized condition.
