Fig. 5: IR spectroscopy characterization.
From: Protein control of photochemistry and transient intermediates in phytochromes
a, b, c Representative structures of cluster 0, 1, and 2, respectively. d IR spectra of the different clusters of Lumi-R and the Pr state. We have used 18, 26, and 13 configurations belonging to clusters 0, 1, and 2, respectively. The sticks represent the carbonyl COD stretch. All spectra are normalized to the highest peak of Cl2. e In the upper panel, the averaged spectrum of the Lumi-R was represented together with that of the Pr state. In the lower panel, the theoretical (calc.) and experimental36 (exp.) early Lumi-R − Pr difference spectra were represented (a shift of 8 cm−1 has been applied to the calculated spectrum to properly compare with the experimental one measured in D2O. Source data are available in the Zenodo repository89.
