Fig. 1: Hairpin-containing slncRNA molecules phase separates in vitro.
From: Formation of synthetic RNA protein granules using engineered phage-coat-protein -RNA complexes
a Construct diagram depicting in vitro transcription of hairpin containing slncRNA molecules used and their gelation under suitable conditions. b Microscopy images showing dependence of structure morphology on the number of binding sites in the slncRNA. PP7-3x results in no visible puncta, while other slncRNAs shows multiple isolated puncta and additional larger fluorescent structures. All scale bars are 10 µm. c Sample image of a PCP-8x granule reaction depicting a larger structure. Scale bar is 10 µm. d Violin plots of median condensate fluorescence of slncRNA-only condensates. e Boxplots showing area (in pixels) of localized condensates, showing that longer slncRNA molecules typically result in larger condensates. Asterisks denote statistical significance at the 5% level according to a two-sample t test. (p-values: PCP-4x vs. PCP-8x: 4e-4; PCP-4x vs. PCP-14x/MCP-14x: 0.046; PCP-4x vs. PCP-3x/MCP-3x: 0.043; PCP-8x vs. PCP-4x/MCP-4x: 0.025; PCP-4x/MCP-4x vs. PCP-14x/MCP-14x: 0.031). On each box, the central mark indicates the median, and the bottom and top edges of the box indicate the 25th and 75th percentiles, respectively. The value for ‘Whisker’ corresponds to ±1.5 IQR (interquartile rate) and extends to the adjacent value, which is the most extreme data value that is not an outlier. The outliers are plotted individually as plus signs. f Poisson function fits for the median fluorescence intensities of the slncRNA granules. g K0 estimates calculated from the Poisson fits, showing a dependence on the number of binding sites in the slncRNA molecule. Source data are provided as a Source data file.
