Fig. 7: In vivo granules present similar dynamics as in vitro.
From: Formation of synthetic RNA protein granules using engineered phage-coat-protein -RNA complexes
a Empirical amplitude distributions gathered from 255 traces in vivo from cells expressing the PCP-4x/QCP-5x slncRNA together with the tdPCP-mCherry protein. b Boxplots depicting burst amplitude distributions (top—positive bursts, bottom—negative bursts). c Boxplots depicting distributions of durations between entry events (green), and durations between shedding events (red). d Boxplot of mean granule fluorescence intensity. e Boxplot of mean cell fluorescence intensity. In (b–e) On each box, the central mark indicates the median, and the bottom and top edges of the box indicate the 25th and 75th percentiles, respectively. The value for ‘Whisker’ corresponds to ±1.5 IQR (interquartile rate) and extends to the adjacent value, which is the most extreme data value that is not an outlier. The outliers are plotted individually as plus signs. f Population intensities of E. coli BL21 cells expressing tdPCP-mCherry with different slncRNAs and different combinations of induction as measured by flow cytometry. Source data are provided as a Source data file.
