Fig. 5: The 3’ UTR of Rasgrp1 aggravates dextran sulphate sodium (DSS)-induced colitis in Il6^+/+ mice.

a, b Weight changes over time of Il6^+/+ mice (a) and Il6^–/– mice (b) after intraperitoneal injection of the indicated molecular (means ± SEM, One-way ANOVA Tukey test, for a: ***p = 0.0001, ***p = 0.0001, in sequence, n = 6 per group; for b: n = 6 per group). c The length of the colons of the Il6^+/+ mice treated as described in (a) (means ± SEM, One-way ANOVA Tukey test, ***p = 0.0001, **p = 0.001, in sequence, n = 6 per group). d Gross appearance of representative colons collected from Il6^+/+ mice (n = 6 per group) treated as described in (a). e ELISA of IL-6 in serum of Il6^+/+ mice treated as described in (a) (means ± SEM, One-way ANOVA Tukey test, ***p = 0.0001, ***p = 0.0001, in sequence, n = 6 per group). f Haematoxylin and eosin (H&E) staining of the colons of the Il6^+/+ mice (n = 6 per group) treated as described in (a). g Immunohistochemistry (IHC) staining for IL-6 protein in the colons of the Il6^+/+ mice (n = 6 per group). h Immunofluorescence costaining for macrophages and IL-6 in the colons of the Il6^+/+ mice (n = 6 per group) treated as described in (a). i The histopathological score in (f) (the means ± SEM, n = 6, Mann-Whitney U, two-side, **p = 0.002, **p = 0.002, in sequence, n = 6 field per group). j The number of IL-6 positive cells per 1000 cells shown in (g) was determined with Image-Pro Plus 6.0 software (the means ± SEM, n = 6, Mann–Whitney U, two-side, **p = 0.002, **p = 0.002, **p = 0.002, in sequence, n = 6 field per group). k The number of IL-6-positive macrophages shown in (h) (means ± SEM, n = 6, Mann–Whitney U, two-side, **p = 0.0001, **p = 0.0001, in sequence, n = 6 field per group). Scale bars represent 50 μm (bottom). All experiments were repeated three times. ns, not significant; *p < 0.05, **p < 0.01, ***p < 0.001.