Fig. 2: Increasing stromal Foxf2 suppresses RM-1 growth in immunocompetent hosts.

a Representative image of RM-1 tumors grown subcutaneously with control (Ctrl) and Foxf2-expressing mouse prostate stromal cells in male SCID/Biege hosts. Dot plot shows means ± s.d. of tumor weight. Bar = 1 cm. N = 8 tumors per group. Two-sided unpaired t-test. b Representative image of RM-1 tumors grown subcutaneously with control and Foxf2-expressing mouse prostate stromal cells in male C57BL/6 hosts. Dot plot shows means ± s.d. of tumor weight. Bar = 1 cm. N = 8 tumors per group. Two-sided unpaired t-test. Co-immunostaining of Keratin 8 (K8) and cleaved caspase 3 (CC3) (c) and K8 and BrdU (d). Dot plots show means ± s.d. of CC3⁺ (c) and BrdU⁺ (d) cells from 5 tumors per group. Each dot represents average value from 20 fields per tumor. Bars = 25 μm. (e-g) FACS plots of T cells (e), myeloid derived suppressor cells (MDSC) (f), and macrophages (MØ) (g) in RM-1 tumors grown with control and Foxf2-expressing mouse prostate stromal cells. Dot plots show means ± s.d. of percentages of CD45⁺, CD3⁺ and CD8⁺ cells (e), monocytic (M-) and polymorphonuclear (PMN-) MDSC cells (f) and MHCII⁺CD206^- M1 and CD206⁺ M2 cells (g). N = 7 tumors per group. two-sided unpaired t-test. h qRT-PCR of gene expression in CD45⁺CD11b⁺Gr-1⁺ myeloid cells FACS-sorted from RM-1 tumors grown with control and Foxf2-expressing stromal cells. Data represent means ± s.d. from 4 tumors per group. Two-sided unpaired t-test. i ELISA of Tnfα and Infγ in lysates of RM-1 tumors grown with control and Foxf2-expressing stromal cells. Data represent means ± s.d. from 8 tumors per group. Two-sided unpaired t-test. j Scatter plots show correlation between expression level of FOXF2 and that of CD8A (left panels), CD8 T-cell signature (middle panels), and cytotoxic T-cell signature (right panels) in prostate cancer datasets by TCGA (upper panels, N = 498) and Erho et al. (lower panels, N = 546) by Pearson correlation analysis. Source data are provided as a Source Data file.