Fig. 5: Sentinel node resection, or irradiation, reduces regional recurrence.

A Overall survival, local progression free survival and regional progression free survival curves for mice treated with a late neck resection depicted in Supplementary Fig. 4E (Tumor+LN removal, n = 6; Tumor removal, n = 6; Sham surgery, n = 6; and no surgery, n = 4). B Schematic of experimental design to look at rates of regional progression in the setting of tumor only SBRT and anti-CD25 with or without a distant tumor (n = 10 for buccal and flank tumor group and n = 15 for only buccal tumor group). Created with BioRender.com. C Overall survival, local progression free survival, and regional progression free survival curves for mice implanted with a buccal tumor or a buccal tumor and a flank tumor. 95% C.I. is shown in dotted lines (n = 25 mice). D Regional progression free survival and cancer free survival of mice treated with ENI, tumor only SBRT, or Sentinel Lymph node SBRT (ENI, n = 10; tumor only SBRT, n = 10, and Sentinel LN SBRT, n = 10). Experimental design is depicted in Supplementary Fig. 7F. E Tumor growth curves for mice treated with or without sentinel lymph node removal (n = 15 for both groups). To the right, the individual curves are shown. F Overall survival, local progression free survival, and regional progression free survival curves are shown for mice treated with sentinel lymph node removal (n = 15 for both groups). A log-rank (Mantel–Cox) test was conducted to determine the survival difference between treatment groups. Significance was determined if the p-value was <0.05*, <0.01**, and <0.001***. Source data are provided as a Source Data file. p-values are indicated for figures E. ***0.0001, and G. **0.0021, **0.0018.