Fig. 4: The AD-associated PKCα M489V mutation exacerbates Aβ-induced synaptic depression. | Nature Communications

Fig. 4: The AD-associated PKCα M489V mutation exacerbates Aβ-induced synaptic depression.

From: Enhanced activity of Alzheimer disease-associated variant of protein kinase Cα drives cognitive decline in a mouse model

Fig. 4

a Experimental design of dual-patch whole-cell recordings (see Material and Methods section). b Dot plot showing the individual excitatory post-synaptic currents (EPSCs) in CT100 infected versus uninfected CA1 hippocampal neurons from WT (blue open circles, N = 26) and M489V (red open circles, N = 29) mice. Group averages indicated by filled circles, error bars indicate SEM. c Bar graph of dual-patch recordings for indicated groups (same data as in b, n = 26 wt and n = 29 M489V CA1 hippocampal neurons); responses were normalized to uninfected controls (solid bars) and graph depicts the mean ± SEM. Paired two-tailed t tests were used to assess statistical significance of dual-patch recordings and obtain p values. To compare CT100-induced depression in WT versus M489V mice, two-way ANOVA was performed (p < 0.0001), followed with Newman–Keuls multiple comparison post hoc test to determine the p value. d Representative traces (mean of at least 40 consecutive trials) obtained from evoked AMPA-receptor-mediated responses from WT and M489V CA1 hippocampal neurons. Source data are provided in the Source Data file.

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