Fig. 3: sf-MSSRn extends spatial resolution in confocal microscopy.
a Comparison of confocal and sf-MSSRn reconstruction (n = 0–3), applied to a spaced fluorescent line pattern. Central lines are gradually being separated by steps of 30 nm (0 nm, 30 nm, 60 nm, …, 390 nm). b Results of confocal and sf-MSSRn reconstruction (n = 0–3) of line patterns separated at 0 nm, 30 nm, 60 nm, 120 nm, 240 nm and 390 nm. c Average profiles of images obtained in b. Images were acquired using a Plan-Apochromat 63x/1.4 Oil immersion objective, exciting the Argo-SIM micropattern E with a 405 nm laser and detecting the fluorescence in the range 420–480 nm (Zeiss LSM880). The pixel size was 44 nm. Images in (a) a (b) correspond to the ensemble average of 31 consecutive sections (width = 1 µm) along the Argo-SIM micropattern E.
