Fig. 7: mDia1- and Arp 2/3-nucleated F-actin networks differentially constrain myosin thick filaments.
From: F-actin architecture determines constraints on myosin thick filament motion
a Filament maximum speed (vmax) as a function of myosin thick filament length (Lmyo) on networks of different architectures and density. Green dashed curves are ‘guide to eye’ for the inverse relationship. Myosin thick filaments are considered as immobile (pixel resolution = 0.1 μm) below the red dashed lines. b Absolute value of the slope of mean speed vEE vs Lmyo for all conditions. (*p < 0.05, ****p < 0.0001, and ns is not significant. p(74 nM Arp & 0.74 nM Arp) = 0.0148, p(74 nM Arp & 83 nM mDia1) = 4.34e-5, p(74 nM Arp & 830 nM mDia1) = 0.0172, p(no nucleator & no nucleator + blebb) = 0.0229. Nmyo = 3444, 3668, 2258, 2588, 668, 1851, and 775 for each condition from left to right. N = 3 independent experiments for each condition. Two tailed t test. Error bars represent s.d. of the mean. Source data are provided as a Source Data file).
