Fig. 5: Neutrophil-specific deficiency of PD-L1 facilitates neutrophil migration from the bone marrow into the kidney of C. albicans-infected mice.
From: PD-L1 negatively regulates antifungal immunity by inhibiting neutrophil release from bone marrow
a The percentage of PD-L1+ neutrophils in bone marrow and kidney of CD274fl/fl and CD274fl/flMRP8Cre/+ mice after infection with C. albicans SC5314 (2 × 105 CFU/mouse) on Day 4. b Neutrophil tracking assay in the bone marrow and kidney of CD274fl/fl and CD274fl/flMRP8Cre/+ mice, which were intravenously infection with C. albicans SC5314 (2 × 105 CFU/mouse) for indicated days. PE-labeled anti-CD45 (10 μl) were microinjected into one tibia for 12 h before scarification at each indicated time. c–e The percentage of neutrophils in bone marrow and kidney (c), ELISA quantification of CXCL1 and CXCL2 in bone marrow, serum and kidney (d), and survival and kidney fungal burden (e) of CD274fl/fl and CD274fl/flMRP8Cre/+ mice, which were intravenously infection with C. albicans SC5314 (2 × 105 CFU/mouse) for the indicated days. Data were presented as mean ± SD; n = 5 (a–e), n = 10 (e) biological independent samples. Data were analyzed by unpaired two-sided Student’s t test in a–e or two-sided log rank (Mantel–Cox) tests in e. Source data are provided as a Source data file.
