Fig. 2: Physiological and metabolic changes during hypothermia evoked by chemogenetics and surface cooling.
a, b The TBAT and corresponding Tcore during hypothermia were measured in mice (n = 3 mice). Hypothermia was produced in Vglut2-Cre mice injected with Gq-DREADD-AAV for 3 weeks and followed by CNO i.p. injection (a). Hypothermia was induced in C57BL/6J mice by surface cooling (b). c The numbers of tremors were recorded (F(1, 12) = 155.20, P < 0.0001). d The blood levels of norepinephrine (F(4, 25) = 157.1, P < 0.0001) and (e) glucose (F(4, 20) = 16.97, P < 0.0001) were measured. f The respiration rate was calculated (F(4, 25) = 157.1, P < 0.0001). Statistical analyses used for c–f were 1ANOVA with Tukey’s post hoc test for the C57BL/6J group and an unpaired two-tailed t test for the Vglu2-Cre-CNO mice. VO2 consumption (g), RER (h), and heat level (i) were measured using indirect calorimetry (n = 5 mice). The quantifications of these measurements are shown in Supplementary Fig. 2i–k. All data were mean ± s.e.m. (n = 5 mice). Source data are provided as a Source Data file.
