Fig. 3: Experimental proof of principle.
From: Pre-equilibrium biosensors as an approach towards rapid and continuous molecular measurements
a Normalized biolayer interferometry (BLI) binding data for two monoclonal antibodies, mAb1 (red) and mAb11 (orange), exposed to 20 nM TNFα from 0–300 s and 0 nM TNFα from 300–600 s. Black lines represent digitally low-pass filtered data. The target concentration was estimated using the pre-equilibrium TEA (solid lines) as well as the conventional inverse-Langmuir (dashed lines) for b mAb1 and c mAb11 data. Solid black lines represent the inverse-Langmuir target estimates based on the fraction of bound receptors at equilibrium.
