Fig. 5: GAC signal correlates with cardiac aging and function decline.
From: A Glb1-2A-mCherry reporter monitors systemic aging and predicts lifespan in middle-aged mice
a Representative images showing mCherry fluorescence and anti-mCherry staining in heart sections from young (3 months, for IF and IHC) and old (23 months, for IF; 16 months, for IHC) Glb1+/m mice. Scale bar, 50 µm. b Comparison of thickness of systole/diastolic left ventricle anterior wall (LVAWs/d) and systole/diastolic LV posterior wall (LVPWs/d) between Glb1+/m mice with high (H, n = 5) and low (L, n = 15) GAC signal and between middle-aged (MA, n = 20) and late-aged (LA, n = 20) Glb1+/m mice, as determined by echocardiography. Representative echocardiographic images of H (top) and L (middle) group mice are shown on the far right. c Left ventricle end diastolic volume and end systolic volume in Glb1+/m mice grouped by GAC signal (H, n = 5, and L, n = 15) or chronological age (MA, n = 20, and LA, n = 20). d Left ventricle ejection fraction and fractional shortening in Glb1+/m mice grouped by GAC signal (H, n = 5, and L, n = 14) and chronological age (MA, n = 19, and LA, n = 19). “n” represents number of biological replicates. Data represent the means ± s.e.m. p value was calculated by Student’s t test (two-sided).
