Fig. 6: GAC signal is exponentially enhanced during pathological senescence.
From: A Glb1-2A-mCherry reporter monitors systemic aging and predicts lifespan in middle-aged mice
a Representative images showing whole-body GAC signal in Glb1+/m mice before BLM treatment (B/BLM), and 7 and 14 days after BLM treatment (A/BLM 7D, A/BLM 14D). #35, #38, #39: 16 months, female, Glb1+/m mice; C: 16 months, female, Glb1+/+ mice; #34, unrelated mice. b Quantification of GAC signal in a. n = 3 mice. c GAC signal and anti-SMA staining in lung section from Glb1+/m mice 14 days after treatment with BLM and quantification of signal intensity. Tissues from n = 3 mice were quantified in each group. Over 100 cells per group were counted. Scale bar, 50 µm. d Masson’s trichrome staining of lung section of Glb1+/m mice 14 days after treatment with BLM or untreated (CON) and quantification of positive area. Tissues from three mice were quantified in each group. Scale bar, 100 µm. “n” represents number of biological replicates. Data represent the means ± s.e.m. p value was calculated by Student’s t test (two-sided).
