Fig. 7: Anabolic and injury responses of Dlx5-creER⁺ perichondrial cell-derived marrow stromal cells.

a–c Differential responses of marrow stromal cells derived from Dlx5⁺ perichondrial cells and Fgfr3⁺ chondrocytes to intermittent administration of parathyroid hormone (PTH) (iPTH). (b): Col1a1(2.3 kb)-GFP; Dlx5-creER; R26R^tdTomato (Dlx5^CE-E12.5, left), Col1a1(2.3 kb)-GFP; Fgfr3-creER; R26R^tdTomato (Fgfr3^CE-E12.5, center), and Col1a1(2.3 kb)-GFP; R26R^tdTomato (Control, right) distal femurs, with iPTH or Vehicle for 3 weeks from P21 to P42. Scale bar: 500 µm. (c): Quantification of Col1a1(2.3 kb)-GFP⁺ cells (left) and Col1a1(2.3 kb)-GFP⁺tdTomato⁺ cells (right) in the metaphysis. n = 6 (Dlx5^CE-E12.5), n = 5 (Fgfr3^CE-E12.5), n = 4 (Control) mice. Two-tailed, One-way ANOVA followed by Tukey’s post-hoc test (left). Two-tailed, Mann-Whitney’s U-test (right). Data are presented as mean ± s.d. Exact P value is indicated in the figures. d–f Injury-responsive osteogenesis of marrow stromal cells derived from fetal perichondrial cells (Dlx5^CE-E12.5) and chondrocytes (Fgfr3^CE-E12.5) cells. (e): Reactive osteogenesis induced by bone marrow ablation in Col1a1(2.3 kb)-GFP; Dlx5-creER; R26R^tdTomato (left panels) and Fgfr3-creER; R26R^tdTomato (right panels) femurs at P28 (pulsed at E12.5). These mice underwent surgery at P21. Metaphyseal (upper) and diaphyseal (lower) marrow space after one week of surgery. Scale bar: 500 µm. (f): Quantification of Col1a1-GFP⁺tdTomato⁺ cells in the metaphyseal and diaphyseal bone marrow. Percentage of Col1a1-GFP⁺tdTomato⁺ osteoblasts within Col1a1-GFP⁺ osteoblasts in the metaphysis (left) and diaphysis (right). n = 5 (Dlx5^CE-E12.5), n = 4 (Fgfr3^CE-E12.5) mice. Two-tailed, Mann-Whitney’s U-test. Data are presented as mean ± s.d. Exact P value is indicated in the figures. Source data are provided as a Source Data file.