Fig. 5: Hepatic Gys2 overexpression alleviated glycogen shortage caused by Mettl3 knockout.

a, b Western blotting assay (a) and IHC staining (b) reveal the effect of reconstitution of GYS2. Luc, Luciferase. For figure b, bar = 50 μm. n = 5 animals. Data are presented as mean values +/− SEM. One-way ANOVA was performed to determine a difference between columns. ***p < 0.001. c–e Hepatic glycogen content assay (c) PAS staining (d) and transmission electron microscope pictures (e) of mouse livers with indicated genotypes and a luciferase (Luc) or Gys2-overexpressing adeno-associated virus (AAV). Bar: 50 μm (d), 0.5 μm (e). n ≥ 4 animals. Data are presented as mean values +/− SEM. One-way ANOVA was performed to determine a difference between columns in figure (c) and (d). ***p < 0.001. f Serum glucose level of indicated groups of mice in free diet. n ≥ 4 animals. Data are presented as mean values +/− SEM. One-way ANOVA was performed to determine a difference between columns. *p < 0.05; **p < 0.01. g Blood glucose level of indicated groups of mice after prolonged fasting. n ≥ 4 animals. Data are presented as mean values +/− SEM. One-way ANOVA was performed to determine a difference between columns. *p < 0.05; ***p < 0.001. h Exhaustive swimming assay of indicated groups of mice. n ≥ 6 animals. Data are presented as mean values +/− SEM. One-way ANOVA was performed to determine a difference between columns. ***p < 0.001. Source data are provided as a Source data file.