Fig. 6: Mutational analysis of the outer leaflet phospholipid binding site. | Nature Communications

Fig. 6: Mutational analysis of the outer leaflet phospholipid binding site.

From: Open-channel structure of a pentameric ligand-gated ion channel reveals a mechanism of leaflet-specific phospholipid modulation

Fig. 6

a ELIC5 CA structure showing POPG (yellow) at the outer leaflet site and residues (dark blue) targeted by mutagenesis. b Tl+ flux rates of mutants in response to 10 mM propylamine for no-exchange (red) and exchange (black, 25 mol% POPG in the outer leaflet) conditions (n = 3). Source data are provided as a source data file. c Weighted time constant of desensitization in response to 10 mM propylamine for no-exchange (red) and exchange (black) conditions (n = 4 for WT, n = 3 for all mutants). d Same as c showing peak rates of Tl+ flux in response to 10 mM propylamine for no-exchange (red) and exchange (black) conditions (n = 4 for WT, n = 3 for all mutants). All data are shown as mean ± s.e. for (n) independent experiments. Statistical analysis was performed using a paired, two-sided T-test.

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