Fig. 2: High cholesterol downregulates the expression of LRP3 in cartilage. | Nature Communications

Fig. 2: High cholesterol downregulates the expression of LRP3 in cartilage.

From: Cholesterol-induced LRP3 downregulation promotes cartilage degeneration in osteoarthritis by targeting Syndecan-4

Fig. 2

a Heatmap of mRNA-seq analysis in cartilage tissue of HCD and RD mice. b Filipin staining to detect cholesterol in rat chondrocytes treated with cholesterol (0, 5, and 50 μg/ml). c Quantification of mRNA levels for LRP1, LRP2, LRP3, LRP4, LRP5, and LRP6 in rat chondrocytes treated with cholesterol (50 μg/ml) (n = 4, two-tailed Student’s t-test). d Quantification of LRP3 mRNA (n = 4, one-way ANOVA) and protein levels in rat chondrocytes treated with cholesterol (50 μg/ml) and/or TNF-α (20 ng/ml). e IHC staining for LRP3 in HCD mice and RD mice subjected to Sham or ACLT operation. Quantification of LRP3-positive cells in mouse knee joint cartilage tissues (n = 5, one-way ANOVA). f IHC staining for LRP3 in human cartilage explants treated with cholesterol (50 μg/ml) and/or TNF-α (20 ng/ml). Quantification of LRP3-positive cells in human knee joint cartilage tissues (n = 5, one-way ANOVA). Data are shown as the mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001. n indicates the number of biologically independent samples, mice per group, or human specimens.

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