Fig. 5: Overexpression of LRP3 in cartilage attenuates OA progression. | Nature Communications

Fig. 5: Overexpression of LRP3 in cartilage attenuates OA progression.

From: Cholesterol-induced LRP3 downregulation promotes cartilage degeneration in osteoarthritis by targeting Syndecan-4

Fig. 5

a Scheme of the OA treatment with intra-articular injection of Lv-Lrp3 in rats. b Representative images of safranin O-fast green, COLII, and LRP3 IHC staining of Normal, Sham, Vehicle, and Lv-Lrp3 treatment rats at 6 w, insets indicate the regions shown in the enlarged images (n = 8). c Quantification of LRP3-positive cells and IOD value for COLII in Normal, Sham, Vehicle, and Lv-Lrp3 treatment rats (n = 5, one-way ANOVA). d The corresponding OARSI scores of Normal, Sham, Vehicle, and Lv-Lrp3 treatment rats at 6 w (n = 8, one-way ANOVA). e The pain response of rats was analyzed using a hot plate and weight-bearing tests (n = 10). f Quantification of mRNA levels for Col2a1, ACAN, Sox9, Lrp3, Mmp13, and Adamts5 in articular cartilage obtained from Sham, Vehicle, and Lv-Lrp3 (high dose group) treatment rats (n = 5, one-way ANOVA). g Micro-CT Coronal and 3D view and the quantitative micro-CT analysis of tibial subchondral bone with trabecular bone volume per total volume (BV/TV) and trabecular bone pattern factor (Tb. Pf) in Sham, Vehicle, and Lv-Lrp3 (high dose group) treatment rats (n = 4, one-way ANOVA). h Biomechanical properties of cartilage samples from Normal, Sham, Vehicle and Lv-Lrp3 treatment rats at 6 w were assessed using a nanoindentation test (n = 5, one-way ANOVA). Data are shown as the mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001. n indicates the number of biologically independent samples or mice per group.

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