Fig. 2: The enzyme kinetics of LbNOX, EcSTH, and EcSTH-LbNOX.
From: Identification of purine biosynthesis as an NADH-sensing pathway to mediate energy stress
a SDS-PAGE of purified recombinant EcSTH, LbNOX, and EcSTH-LbNOX. b The activity of LbNOX in relation to NADH concentration (n = 3 biologically independent samples). c The activity of EcSTH in relation to NADPH concentration (with 400 µM thio-NAD+) (n = 3 biologically independent samples). d The activity of EcSTH in relation to Thio-NAD+ concentration (with 400 µM NADPH) (n = 3 biologically independent samples). e The activity of EcSTH-LbNOX in relation to NADPH concentration (with 400 µM thio-NAD+) (n = 3 biologically independent samples). f The activity of EcSTH-LbNOX in relation to Thio-NAD+ concentration (with 400 µM NADPH) (n = 3 biologically independent samples). g Simultaneous measurement of the reduction of thio-NAD+ and the oxidation of NADPH by EcSTH, LbNOX, and EcSTH-LbNOX (with 400 µM thio-NAD+ and NADPH respectively) (n = 3 biologically independent samples). All experimental data were verified in at least two independent experiments. Error bars represent mean ± SD. Source data are provided as a Source Data file.
