Fig. 5: LATS1 interacts and cooperates with NCOR1.

a Significantly differentially expressed genes (FC > 1.5, p-value < 0.05) in each of the indicated comparisons were analyzed for “Upstream regulators” using IPA (QIAGEN). NCOR1 activity in the different conditions was determined by “Activation Z-score” and/or directionality of expression of genes repressed or activated by NCOR1, as determined by IPA. Source data are provided as a Source Data file. b RT-qPCR analysis of representative NCOR1-ERα repressed genes in RNA from WT or Lats1-CKO PyMT cells. Values were normalized to Hprt; WT values were set as 1.0. Average ± SE of three independent cell lines of each genotype. An unpaired t-test was used to calculate significance. Source data are provided as a Source Data file. c Integrative Genomics Viewer (IGV) snapshots depicting the ATAC-seq signal of representative NCOR1-ERα repressed genes in WT luminal (WT) compared to Lats1-CKO luminal (L1) cells. For each WT-L1 comparison, the Y-axis scale is identical. The associated RefSeq gene structure (or enhancer region) is presented below the tracks. d RT-qPCR analysis of representative NCOR1-repressed genes in RNA from MDA-MB-468 cells harboring vector control or inducible LATS1, following 48 h of doxycycline induction. Values were normalized to HPRT; vector control values were set as 1.0. Average ± SE of five biological replicates. Unpaired t-test was used to calculate significance. Source data are provided as a Source Data file. e WT and Lats1-CKO PyMT cells were transfected with control siRNA (siCont) or siRNA against Ncor1 (siNcor1). Three days after transfection, an additional dose of siRNA was administered. Three days later, cells were harvested for RT-qPCR analysis of the indicated NCOR1-repressed genes. Values were normalized to Hprt. Average ± SE of three biological replicates. One-way ANOVA was used to calculate significance. Source data are provided as a Source Data file. f Lats1-CKO PyMT cells, stably transduced with MYC-tagged mouse Lats1 or vector control, were subjected to immunoprecipitation with antibodies against MYC-tag (9E10) or LATS1, followed by Western blot analysis with the indicated antibodies. 2.5% of each lysate was run as “input”. β-ACTIN served as loading control for input. Representative blot of two biological repeats. g WT and Lats1-CKO cells, transiently transfected with GFP-tagged mouse Lats1, were subjected to immunofluorescent staining of NCOR1 and GFP. Arrows denote cells expressing transfected GFP-tagged mouse LATS1. Scale bar = 100 μm. Representative images of four biological repeats.