Fig. 1: Pear1 deficiency exacerbated PF by direct regulation of mesenchymal cells function.

a Forced vital capacity (FVC) was measured for evaluating lung function of 12-month-old WT mice and Pear1^−/− mice (n = 10 mice in WT group; n = 9 mice in Pear1^−/− group). WT, wild type. b Masson staining of lung tissue in 12-month-old WT mice and Pear1^−/− mice. The collagen area (green) was calculated (n = 10 mice per group) (scale bars, 50 μm). c FVC of WT and Pear1^−/− mice was measured on day 21 after bleo administration (n = 9 mice in WT group; n = 5 mice in Pear1^−/− group). d Survival curves of WT and Pear1^−/− mice induced by 1.5 µg/g or 2 µg/g bleo through endotracheal atomization (n = 10 mice per group). e Representative images of masson staining on lung sections from WT mice and Pear1^−/− mice on day 21 after bleo administration. The collagen area (green) was calculated (n = 10 mice per group) (scale bars, 50 μm). f The proportion of leukocytes (CD45⁺), endothelial cells (CD31⁺), epithelial cells (CD326⁺) and Pdgfra⁺ mesenchymal cells (MSC, CD45⁻CD31⁻CD326⁻) were analyzed in the lung tissue of Pear1^−/− mice with or without treatment of bleo (n = 5 mice in PBS group; n = 8 mice in bleo group). g PEAR1⁺ cell proportion in Pdgfra⁺ mesenchymal cells (Pdgfra⁺CD45⁻CD31⁻CD326⁻) from WT mice lung treated with or without bleo was investigated by flow cytometry. h FVC was measured of Pear1^f/f and Col1a2-Cre^ERPear1^f/f mice on day 21 after bleo administration (n = 9 mice in Pear1^f/f group; n = 5 mice in Col1a2-Cre^ERPear1^f/f group). i Survival curves of Pear1^f/f and Col1a2-Cre^ERPear1^f/f mice induced by 1.5 µg/g bleo through endotracheal atomization (n = 15 mice in Pear1^f/f group; n = 17 mice in Col1a2-Cre^ERPear1^f/f group). j Representative images of masson staining on lung sections from Pear1^f/f and Col1a2-Cre^ERPear1^f/f mice on day 21 after bleo administration. The collagen area (green) was calculated (n = 9 mice in Pear1^f/f group; n = 5 mice in Col1a2-Cre^ERPear1^f/f group) (scale bars, 50 μm). For a–c, e, h, j, two-tailed t test was used. For f, one-way ANOVA was used. Data are presented as mean ± SD. Source data are provided as a Source data file.