Fig. 6: ProQ/FinO domain proteins bind transcriptional terminator structures to regulate RNA-RNA interactions. | Nature Communications

Fig. 6: ProQ/FinO domain proteins bind transcriptional terminator structures to regulate RNA-RNA interactions.

From: Structural basis for recognition of transcriptional terminator structures by ProQ/FinO domain RNA chaperones

Fig. 6

a Many ProQ/FinO domain proteins, such as RocC, facilitate in trans RNA association between sRNAs and target mRNAs. The ProQ/FinO domain specifically binds the transcriptional terminator of the RocR sRNA, stabilizing it against degradation. Key to RNA-RNA association is the recognition of the sRNA seed sequence with its complementary region (the RocR box) in the target mRNA. The C-terminal region of RocC is required to facilitate the recognition and translational repression of target mRNAs such as comEA. b Many plasmid-encoded ProQ/FinO domain proteins, such as FinO, regulate in cis sense-antisense RNA interactions. Similar to RocC, the ProQ/FinO domain of FinO also stabilizes the antisense RNA FinP against degradation. Initial RNA-RNA interactions are thought to involve loop–loop kissing interactions, which then proceed to duplex formation between the two RNAs, resulting in translational repression of the traJ mRNA target. In this case, the flexible N-terminal region of FinO is thought to be key in facilitating RNA-RNA interactions.

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