Fig. 3: Targeting effects of LH NP and TLH NP in pregnant rats with stenosis-induced DVT. | Nature Communications

Fig. 3: Targeting effects of LH NP and TLH NP in pregnant rats with stenosis-induced DVT.

From: Functionally integrating nanoparticles alleviate deep vein thrombosis in pregnancy and rescue intrauterine growth restriction

Fig. 3

a A schematic diagram showing the establishment of a stenosis-induced DVT model in pregnant rats. b, c Ex vivo fluorescence images (b) and quantitative data (c) show the accumulation of Cy5-LH NP or Cy5-TLH NP in normal veins or veins with stenosis-induced thrombosis at 4 h after i.v. injection in pregnant rats at G10. d–f Immunofluorescence analysis of the co-localization of Cy5-LH NP or Cy5-TLH NP with vWF-positive endothelial cells (d), MPO-positive neutrophils (e), and CD61-positive platelets (f) in cryosections of left iliac veins of DVT rats. Pregnant rats (at G10) with stenosis-induced DVT were administered with Cy5-LH NP or Cy5-TLH NP via i.v. injection. At 4 h after administration, rats were euthanized and left iliac veins were isolated for analysis. Scale bars, 40 μm. g Ex vivo imaging of placentas and fetuses at 4 h after i.v. injection of Cy5-LH NP or Cy5-TLH NP in normal or DVT pregnant rats (at G15). h Microscopic observation of placental sections. Upper panel, bright field images; lower panel, fluorescence images. Scale bars, 2 mm. In all cases, pregnant rats with thrombosis were treated with the same volume of saline in the saline group. Data in b, d–h are representative of six independent samples. Data in c are mean ± s.d. (n = 6 independent samples). Statistical significance was assessed by one-way ANOVA with post hoc LSD tests. ***p < 0.001. Source data are provided as a Source Data file.

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