Fig. 1: Frameshift mutation of RIG-I exacerbates colitis-associated cancer.
From: Mutant RIG-I enhances cancer-related inflammation through activation of circRIG-I signaling
a Strategy for generation of Rig-ifs/fs mice. The amino acid and nucleic acid sequence of frameshift Rig-i mutation were shown. b Immunoblot analysis of protein level of RIG-I in MEFs from 6-8-week-old male indicated (wild-type (WT) and Rig-ifs/fs) mice with or without VSV infection detected by RIG-I antibody against its N terminal region. Data are collected from 2 independent experiments. c The measurement of body weight of 6-8-week-old indicated mice with AOM/DSS treatment (n = 8 mice, mean ± s.e.m.). d-e The level of CA19-9 (d) and CEA (e) in indicated mice serum was measured at day 89 in AOM/DSS-induced Colitis-Associated Cancer (CAC) model (n = 8 mice, mean ± s.e.m., **P = 0.0020, ***P = 0.0005, two-tailed unpaired Student’s t-test). f Representative pictures of colon tumors from indicated mice in AOM/DSS-induced Colitis-Associated Cancer (CAC) model. g-h The tumor number (g) or tumor load (h) in the whole colon was measured (n = 4 mice, mean ± s.e.m., **P = 0.0028, ****P < 0.0001, two-tailed unpaired Student’s t-test). i Representative H&E (Hematoxylin-eosin) staining pictures of colon tumors (n = 2 mice). The scale bars represent 500 μm. j-k Macroscopic evaluation (j) and cell number (k) of spleen in AOM/DSS-induced Colitis-Associated Cancer (CAC) model (n = 4 mice, mean ± s.e.m., *P = 0.0179, two-tailed unpaired Student’s t-test). l-m Flow cytometric analysis of frequency of CD4+ T cell, CD8+ T cell and B cell subsets in spleen (n = 5 mice, mean ± s.e.m., **P = 0.00544, ***P = 0.0003, ****P < 0.0001, two-tailed unpaired Student’s t-test) (l) and lamina propria mononuclear cells (LPMC) (n = 5 mice, mean ± s.e.m., ****P < 0.0001, two-tailed unpaired Student’s t-test) (m) isolated from wild-type (WT) and Rig-ifs/fs mice treated with AOM/DSS. Source data are provided as a Source Data file.
