Fig. 2: Rig-ifs/fs mice display increased susceptibility to experimental colitis.
From: Mutant RIG-I enhances cancer-related inflammation through activation of circRIG-I signaling
a Survival analysis of described mice (wild-type (WT), n = 11 mice; Rig-ifs/fs, n = 9 mice; **P = 0.0029, Log-rank (Mantel-Cox) test). b-c Macroscopic evaluation (b) and colon length (c) of indicated mice treated with 2 % (weight/volume) DSS for 8 days (n = 4 mice, mean ± s.e.m., **P = 0.0036, two-tailed unpaired Student’s t-test). d Representative H&E staining pictures of colon tissues from indicated mice treated with 2 % (weight/volume) DSS (n = 2 mice). The scale bars represent 250 μm. e A graphic model of bone marrow transplantation assay. f Survival analysis of indicated mice transplanted with WT bone marrow in DSS model (n = 6 mice, *P = 0.0326, Log-rank (Mantel-Cox) test). g-h Macroscopic evaluation (g) and colon length (h) of indicated mice transplanted with WT bone marrow in DSS model (n = 6 mice, mean ± s.e.m., ****P < 0.0001, two-tailed unpaired Student’s t-test). i Body weight (relative to initial weight, set as 100%) of indicated mice transplanted with WT bone marrow in DSS model (n = 6 mice, mean ± s.e.m., *P = 0.0338, **P = 0.0073, P < 0.0001, two-tailed unpaired Student’s t-test). j Representative H&E staining pictures of indicated mice colon in DSS model (n = 2 mice). The scale bars represent 1000 μm. k Gene Set Enrichment Analysis (GSEA) of differentially expressed genes in colon from indicated mice treated with DSS (n = 3 mice). ES, enrichment score; NES, normalized enrichment score. l Survival analysis of indicated mice (n = 5 mice, *P = 0.0112, Log-rank (Mantel-Cox) test). m GSEA of the differentially expressed genes in liver from indicated mice treated with LPS for 24 hours (n = 2 mice ES, enrichment score; NES, normalized enrichment score. n-o RT-qPCR analysis of the indicated mRNA levels in liver from indicated mice treated with LPS (n = 3 mice, mean ± s.e.m., **P = 0.0044 (Isg15), **P = 0.0066 (Tnfα), two-tailed unpaired Student’s t-test). The primers used for quantitative real-time PCR have been deposited in Supplementary Data 5. Source data are provided as a Source Data file.
