Fig. 5: Clinical analysis of circRIG-I expression in colon cancers.
From: Mutant RIG-I enhances cancer-related inflammation through activation of circRIG-I signaling
a Sequence of human circRIG-I junction site was shown in the colon cancer sample. Data are collected from 2 independent experiments. b RT-qPCR analysis of level of circRIG-I in colon cancers and their matched adjacent normal tissues (n = 100 patients, mean ± s.e.m., **** P < 0.0001, two-tailed paired Student’s t-test). c RT-qPCR analysis of level of circRIG-I between ulcerative colon cancer (n = 44 patients) and polypoid (n = 30 patients) colon cancers (mean ± s.e.m., *P = 0.0296, two-tailed unpaired Student’s t-test). d RT-qPCR analysis of level of circRIG-I in colon cancers with different stage (T1/T2, n = 14 patients; T3, n = 76 patients; T4, n = 10 patients; mean ± s.e.m., *P = 0.0247, two-tailed unpaired Student’s t-test). e RT-qPCR analysis of level of circRIG-I in colon cancers with different age (<65, n = 52 patients; ≥ 65, n = 48 patients; mean ± s.e.m., **P = 0.00635, two-tailed unpaired Student’s t-test). f RT-qPCR analysis of level of circRIG-I between colon adenocarcinoma (n = 87 patients) and mucinous colon adenocarcinoma (n = 13 patients) (mean ± s.e.m., ns, not significant (P > 0.05), two-tailed unpaired Student’s t-test). g RT-qPCR analysis of the relative level of circRIG-I in colon cancers with different gender (male, n = 53 patients; female, n = 47 patients; mean ± s.e.m., ns, not significant (P > 0.05), two-tailed unpaired Student’s t-test). h RT-qPCR analysis of level of circRIG-I in colon cancers with different locations (colon, n = 50 patients; rectum, n = 47 patients; ileocecus, n = 3 patients; mean ± s.e.m., ns, not significant (P > 0.05), two-tailed unpaired Student’s t-test). i RT-qPCR analysis of level of circRIG-I in the inflammatory foci (P) and matched adjacent normal tissues (N) from patients with ulcerative colitis (n = 8 patients; mean ± s.e.m., **P = 0.004326, two-tailed paired Student’s t-test).The primers used for quantitative real-time PCR have been deposited in Supplementary Data 5. Source data are provided as a Source Data file.
