Fig. 6: CircRIG-I enhances host innate immune response.
From: Mutant RIG-I enhances cancer-related inflammation through activation of circRIG-I signaling
a RT-qPCR analysis of Ifnβ and Ifitm2 mRNA levels in wild-type (WT) and Rig-ifs/fs MEFs in presence or absence of circRIG-I (n = 4 cell cultures, mean ± s.e.m., ***P < 0.0001, two-tailed unpaired Student’s t-test). b Validation of the expression of murine circRIG-I in HEK293T cells by PCR assay. Data are collected from 2 independent experiments. c Fluorescence and light field of mock and murine-derived circRIG-I overexpressing HEK293T with VSV-GFP infection. The scale bars represent 200 μm. Data are collected from 2 independent experiments. d Flow cytometric analysis of GFP+ cells from mock and murine circRIG-I overexpressing HEK293T followed infection with VSV-GFP (n = 3 cell cultures, mean ± s.e.m., ****P < 0.0001, two-tailed unpaired Student’s t-test). e-f RT-qPCR analysis of IFNB (n = 3 cell cultures, mean ± s.e.m., ****P < 0.0001, two-tailed unpaired Student’s t-test) (e) and ISGs mRNA levels in mock and murine circRIG-I overexpressing HEK293T cells with VSV infection (n = 3 cell cultures, mean ± s.e.m., **P = 0.0055 (IFITM1), **P = 0.005704 (ISG56), ****P < 0.0001, two-tailed unpaired Student’s t-test) (f). g Immunoblot analysis of phosphorylation level of IRF3 in iBMDM overexpressing mock or murine circRIG-I followed with VSV infection (n = 3 cell cultures, mean ± s.e.m., *P = 0.0186, **P = 0.0011, two-tailed unpaired Student’s t-test). h Subcellular localization of IRF3 in iBMDM overexpressing mock or circRIG-I treated by VSV analyzed by nucleus-cytoplasm extraction (n = 3 cull cultures, mean ± s.e.m., **P = 0.0065 (12 h), **P = 0.0090 (24 h), two-tailed unpaired Student’s t-test). The primers used for quantitative real-time PCR or PCR assay have been deposited in Supplementary Data 5. Source data are provided as a Source Data file.
