Fig. 8: All-trans retinoic acid alleviates colonic inflammatory lesion of Rig-ifs/fs mice.
From: Mutant RIG-I enhances cancer-related inflammation through activation of circRIG-I signaling
a RT-qPCR analysis of Dhx9 mRNA in MEFs with ATRA treatment (n = 6 cell cultures, mean ± s.e.m., ns, *P = 0.0177 (4 h), *P = 0.0204 (8 h), **P = 0.0015, ***P = 0.0002, two-tailed unpaired Student’s t-test). b-c RT-qPCR analysis of Ddx58 mRNA and pre-mRNA as well as circRIG-I expression in MEFs with ATRA treatment (n = 6 cell cultures, mean ± s.e.m., ns, not significant (P > 0.05), ***P = 0.0001, ****P < 0.0001, two-tailed unpaired Student’s t-test). d RT-qPCR analysis of mRNA and pre-mRNA of Ddx58, circRIG-I expression in wild-type (WT) and Rig-ifs/fs MEFs with ATRA treatment (n = 3 cell cultures, mean ± s.e.m., ns, not significant (P > 0.05), **P = 0.0012 (Ddx58), **P = 0.0016 (WT, circRIG-I), **P = 0.0051 (Rig-ifs/fs, circRIG-I), ***P = 0.0005, two-tailed unpaired Student’s t-test). e Survival analysis of wild-type (WT) and Rig-ifs/fs mice with or without ATRA treatment in DSS model (n = 5 mice, **P = 0.0079 (Rig-ifs/fs vs. Rig-ifs/fs + ATRA), **P = 0.0025 (WT vs. Rig-ifs/fs), Log-rank (Mantel-Cox) test). f-g Colon length (f) and macroscopic evaluation (g) of wild-type (WT) and Rig-ifs/fs mice with or without ATRA treatment in DSS model (n = 3 mice, mean ± s.e.m., *P = 0.0173, **P = 0.0017, two-tailed unpaired Student’s t-test). h Representative H&E staining pictures of colon tissues from wild-type (WT) and Rig-ifs/fs mice with or without ATRA treatment in DSS model. The scale bars represent 1000 μm. Data are collected from 2 independent experiments.The primers used for quantitative real-time PCR have been deposited in Supplementary Data 5. Source data are provided as a Source Data file.
