Fig. 5: CRY2 and CIS1 regulate thermosensory flowering via FLM.
From: CRY2 interacts with CIS1 to regulate thermosensory flowering via FLM alternative splicing
a, c Representative photographs of 24-day-old (a) and 20-day-old (c) plants of the indicated genotypes grown at 22 °C in LD conditions. Scale bar = 5 cm. b and d Number of rosette leaves of the indicated genotypes shown in a (for b) and c (for d). Blue and red circles indicate the data from individual plants. Error bars represent the s.d. in (b) and (d). Lowercase letters indicate statistically significant differences, as determined by one-way ANOVA with Tukey’s multiple comparisons test (P < 0.05). e, f Flowering phenotype of the indicated genotypes grown at 22 °C in LD conditions, reported as the number of rosette leaves at the time of flowering. Blue and red circles indicate the data from individual plants. Error bars represent the s.d., two-sided Student’s t-test was used to calculate P values. g, i Representative photographs of 38-day-old plants grown at 22 °C in LD conditions (g) and 93-day-old plants grown at 16 °C in SD conditions (i). Scale bar = 5 cm. h and j Number of rosette leaves of the indicated genotypes shown in g (for h) and i (for j). Blue and red circles indicate the data from individual plants. Error bars represent the s.d. Lowercase letters indicate statistically significant differences, as determined by one-way ANOVA with Tukey’s multiple comparisons test (P < 0.05). k RT-qPCR results showing the expression of FT and SOC1 in the indicated genotypes. Seedlings grown at 22 °C in LD conditions were collected at zeitgeber 16 (ZT16, 16 h after lights on) for the time course after germination. Error bars, s.d. of three biological replicates. The asterisks indicate a significant difference from Col-0 based on a two-sided Student’s t-test (*P < 0.05, **P < 0.01).
