Fig. 7: RhoA controls SRF protein levels and the expression of brain metastasis and drug resistance genes.

A C2 or R2 cells were cultured over 18 days in the presence of osimertinib on control or laminin-coated plates. Lysates were then subjected to western blotting for the indicated proteins. A representative blot from two independent experiments is shown. B R2 cells expressing the indicated shRNAs were cultured as in A. Lysates were then subjected to western blotting for SRF or tubulin. A representative blot from two independent experiments is shown. C R2 cells expressing the indicated shRNAs were cultured on laminin-coated plates with Dox and serum starved for 12 h. Expression of SERPINE1, FOSL1, and KRT13 were measured by qPCR, normalized to HPRT expression and data was plotted with SD. A representative of two independent experiments is shown. N = 3 technical replicates. D R2 cells expressing the indicated sgRNAs were cultured on laminin-coated plates and treated as in B. Expression of FOSL1 were quantified as in C. N = 3 technical replicates. Data presented as mean +/− SD and representative from 2 independent experiments for sgSRF#2 and 3 independent experiments for sgSRF#1.